Alkaline Lysis Reagent
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Alkaline Lysis Reagent
- To 25 ml water
- add 62.5 µl of 10 N NaOH (final conc is 25 mM)
- 10.0 µl of 0.5 M disodium EDTA (final conc is 0.2 mM)
- (pH should be about 12, but should not have to be adjusted)
- Make fresh every 1-2 months. Keep solution at RT.
- Note: because of the presence of EDTA in the alkaline lysis reagent, you may need to increase the amount of magnesium chloride in your PCR master mix. Your PCR conditions may need to be re-optimized when switching to this method of DNA preparation.
