Whole Tissue
From OpenWetWare
Jump to navigationJump to search
Kirk Lab Protocol
Whole Tissue from Frozen Tissue
1)Add 1 mL of Lysis buffer per 100 mg of tissue to a glass homogenization vile
2)Homogenize at 7500 rpm for 2-3 seconds using mechanical homogenizer – repeat 2 times, or until sufficient
3)Transfer homogenate to 1.5 mL Eppendorf tube
4)Spin at 18,000 x g for 10 minutes at 4° C on Kirk Lab Tabletop Centrifuge
5)Remove supernatant (this is your WHOLE TISSUE LYSATE) and discard pellet
6)If necessary, repeat step 4 and save supernatant to further clean sample
