Protocols Kirk Lab
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==KH Buffer==
| Chemicals | Working solution | 10X solution |
|---|---|---|
| NaCl | 140mM --- 8.8g | 81.8g |
| KCl | 4.8mM --- 0.35g | 3.58g |
| MgSO4 | 1.2mM --- 0.144g | 1.44g |
| NaHCO3 | 4mM --- 0.336g | 3.36g |
| NaH2PO4 | 1.2mM --- 0.144g | 1.44g |
| HEPES | 10mM --- 2.383g | 23.83g |
| Glucose | 12.5mM --- 2.25g | ------ (0.25M Glucose 9g/200mL (4C)) |
| DDW | ------- 1000mL | 1000mL (store at 4C) |
Working solution 10X solution 100mL + DDW 850mL + 0.25M Glucose 50mL
Adjust pH 7.4 and filter, store at 4C
