User contributions for Schumer

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15:4215:42, 12 February 2025 diff hist 0‎ Schumer lab:General ‎ →‎General information current
15:4115:41, 12 February 2025 diff hist +16‎ Schumer lab:General ‎ →‎General information
15:3615:36, 12 February 2025 diff hist −125‎ Schumer lab:General ‎ →‎General information
13:5813:58, 12 February 2025 diff hist +3,084‎ N Schumer lab: HSV transduction ‎ Created page with "HSV transduction in Xiphophorus primary embryo cells Last updated April 2024 A few days before transduction: Passage cells into the plates/flask you’d like to use and maintain until 70-80% confluency. Make sure to passage at least 24 hours prior to transduction to avoid stressing the cells as much as possible. I will try to seed around 100k cells in a few wells of a 12 well plate and let them grow for a few days before infection Day of transduction: 1. Treat cell..." current
13:5113:51, 12 February 2025 diff hist +72‎ Schumer lab: Electroporation ‎No edit summary current
13:4913:49, 12 February 2025 diff hist +516‎ N Schumer lab: Electroporation ‎ Created page with "To electroporate using 100 uL tips: Dissociate and count 600k cells. Pellet and resuspend in 120 uL buffer R. Add 2.4 ug plasmid and mix. Draw up 100uL in the electroporation tip, being careful to avoid bubbles. (Bubbles will cause electrical arcs and kill the cells) This allows for 20 uL overage so that 500k cells and 2 ug plasmid to be in the tip. Electroporate at 1400v, 20ms and 2 pulses. Dispense tip into a flask with regular media + ROCKi. Change media the next d..."
13:4013:40, 12 February 2025 diff hist +106‎ Schumer lab:Wet lab/Sequencing ‎ →‎Cell culture current
12:5312:53, 12 February 2025 diff hist +89‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with FileZilla (External hard-drive needed) current
12:5212:52, 12 February 2025 diff hist +188‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with FileZilla
12:4812:48, 12 February 2025 diff hist +823‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with FileZilla
12:2912:29, 12 February 2025 diff hist 0‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with ftp
12:2512:25, 12 February 2025 diff hist +18‎ Schumer lab:Github and bioinformatics wiki ‎ →‎Bioinformatics topics current
12:0812:08, 12 February 2025 diff hist +214‎ Schumer lab: Download data to Sherlock from Admera ‎No edit summary
11:5611:56, 12 February 2025 diff hist −132‎ Schumer lab:Github and bioinformatics wiki ‎ →‎Bioinformatics topics Tag: Manual revert
11:5511:55, 12 February 2025 diff hist +18‎ Schumer lab:Github and bioinformatics wiki ‎ →‎Bioinformatics topics
11:5411:54, 12 February 2025 diff hist +114‎ Schumer lab:Github and bioinformatics wiki ‎ →‎Bioinformatics topics
11:3611:36, 12 February 2025 diff hist −354‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with ftp
11:2311:23, 12 February 2025 diff hist +48‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with ftp
11:2211:22, 12 February 2025 diff hist +46‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with ftp
11:0411:04, 12 February 2025 diff hist +25‎ Schumer lab: Download data to Sherlock from Admera ‎ →‎Download data with ftp

16:4716:47, 6 February 2025 diff hist +130‎ Schumer lab: Commonly used workflows ‎ →‎For first time use current

13:1713:17, 11 December 2024 diff hist +134‎ N Schumer lab: Quick links document ‎ Created page with "Master organizational link document: https://docs.google.com/document/d/1XeQ6orWeelxR7nkZUbUUIZd5VvZoj9d-WnqGsrJ4PjU/edit?usp=sharing" current
13:1613:16, 11 December 2024 diff hist +61‎ Schumer lab:Github and bioinformatics wiki ‎ →‎Where to find important files and resources?
13:1513:15, 11 December 2024 diff hist +208‎ Schumer lab:General ‎No edit summary

18:2318:23, 26 August 2024 diff hist +1‎ Schumer lab: Upload data from Sherlock to NCBI's SRA ‎ →‎Uploading Data from Sherlock to NCBI's SRA current
18:2318:23, 26 August 2024 diff hist +2‎ Schumer lab: Upload data from Sherlock to NCBI's SRA ‎ →‎Uploading Data from Sherlock to NCBI's SRA
18:2318:23, 26 August 2024 diff hist +639‎ Schumer lab: Upload data from Sherlock to NCBI's SRA ‎ →‎Uploading Data from Sherlock to NCBI's SRA

15:0415:04, 21 August 2024 diff hist +10‎ Schumer lab:Grad program ‎ →‎Courses current
15:0115:01, 21 August 2024 diff hist +288‎ Schumer lab:Grad program ‎ →‎Courses
14:5614:56, 21 August 2024 diff hist +492‎ Schumer lab:Grad program ‎ →‎Courses
11:5211:52, 21 August 2024 diff hist −77‎ Schumer lab:Grad program ‎ →‎Quals
11:5111:51, 21 August 2024 diff hist −1‎ Schumer lab:Grad program ‎ →‎Quals
11:5011:50, 21 August 2024 diff hist −46‎ Schumer lab:Grad program ‎ →‎Quals
11:4611:46, 21 August 2024 diff hist −783‎ Schumer lab:Grad program ‎ →‎Quals
11:4111:41, 21 August 2024 diff hist +3,599‎ Schumer lab:Grad program ‎ →‎Quals
11:4011:40, 21 August 2024 diff hist +250‎ Schumer lab: Commonly used scripts ‎No edit summary current
11:3111:31, 21 August 2024 diff hist +402‎ Schumer lab:Grad program ‎ →‎First year requirements - CMOB
11:2811:28, 21 August 2024 diff hist +6‎ Schumer lab:Grad program ‎ →‎First year requirements - CMOB
11:2711:27, 21 August 2024 diff hist +2,344‎ Schumer lab:Grad program ‎ →‎First year requirements - CMOB
10:4710:47, 21 August 2024 diff hist +1‎ Schumer lab:Grad program ‎ →‎Courses
10:4610:46, 21 August 2024 diff hist −1‎ Schumer lab:Grad program ‎ →‎Courses
10:4610:46, 21 August 2024 diff hist +1‎ Schumer lab:Grad program ‎ →‎Courses
10:4510:45, 21 August 2024 diff hist +67‎ Schumer lab:Grad program ‎ →‎Courses
10:4310:43, 21 August 2024 diff hist +536‎ Schumer lab:Grad program ‎ →‎Courses

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