Schumer lab:Wet lab/Sequencing

Sanger sequencing resources

• Primer design
• Sanger sequencing

Pyrosequencing resources

• Pyrosequencing primer design
• Pyrosequencing primer ordering
• Pyrosequencing at Stanford

RNA extraction and Reverse Transcription

• RNA extraction
• Reverse Transcription

qPCR

• Primer efficiency testing
• Data collection

low coverage whole genome sequencing resources

• DNA extraction
• DNA quantification and dilution
• Library Prep

Illumina sequencing resources

• Which indices should I use?
• Pooling libraries for sequencing
• Submitting libraries for sequencing

HMW DNA Extractions

• NEB Monarch Tissue HMW DNA extraction

Cell culture

• Changing media
• Passaging cells
• Electroporation
• HSV transduction