Schumer lab: Electroporation
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To electroporate using 100 uL tips:
Fill the electroporation chamber with 2 mL of electroporation buffer.
Dissociate and count 600k cells. Pellet and resuspend in 120 uL buffer R. Add 2.4 ug plasmid and mix. Draw up 100uL in the electroporation tip, being careful to avoid bubbles. (Bubbles will cause electrical arcs and kill the cells) This allows for 20 uL overage so that 500k cells and 2 ug plasmid to be in the tip.
Electroporate at 1400v, 20ms and 2 pulses. Dispense tip into a flask with regular media + ROCKi. Change media the next day to remove residual buffer R and ROCKi.
