SBB10Ntbk-JennaKelleher1
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Project Information
29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence
Construction Files
Jenna Kelleher 099 Rep Protein (sbb29)
Jenna Kelleher CA42 Rep Gene (sbb28)
Jenna Kelleher Pre-pro Sequence (sbb42)
Useful Protocols
For SBB28 and SBB29:
Cloning by PCR
Regular Zymo Cleanup
For SBB42:
Eco-Bam Transfer
Small-Frag Zymo Cleanup
For All 3:
EcoRI/BamHI Digest of PCR Products
Zymo Gel Purification
Ligation of EcoRI/BamHI digests
Transformation by heat-shock
Picking of colonies
Macherey-Nagel Miniprep
EcoRI/BamHI Digest of PCR Products
Analytical digests (Mapping)
