Template:SBB-EcoBamXfers
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Eco/Bam Transfer
Set up digest:
- Set up the digest:
For BBb transfers: 7.5uL ddH2O 1 uL NEB2+ATP Buffer 0.5uL EcoRI 0.5uL BamHI 0.5uL miniprepped plasmid
- run temperature program on thermocycler of 1hr at 37 and then 20 min at 80
- Add 0.5uL of predigest vector and 0.5uL of T4 DNA ligase
- Incubate 30 min. at room temp, transform, rescue, plate.