Preparing Reagents

Department of Physics, Willamette University

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Preparing Reagents

Making assay buffer

Making glucose + beta-mercaptoethanol (G/β)

Making creatine phosphokinase (CPK)

Making creatine phosphate disodium salt (PCr)

Making nitrocellulose beaded coverslips

Making 0.1 M ATP stock

Polymerizing and labeling actin