Making CPK

Department of Physics, Willamette University

'

Home        Contact        Lab Members        Publications        Research        Protocols        Pictures       

Making Creatine Phosphokinase (CPK)

• Creatine Phosphokinase – Calbiochem, Cat #: 238395, 5KU, store at -20°C
• Final stock (100x) should be 10 mg/mL stored in 0.1% BSA, 50% GlyOH, and AB conditions.

1. Make ABSA + 50% GlyOH

	5 mL ABSA + GlyOH
10x AB stock	500 uL
10x BSA stock	500 uL
100x DTT stock	50 uL
GlyOH	2.5 mL
ddwater	fill to 5 mL

2. Chill the ABSA + GlyOH at -20°C

3. Add 1.5 mL of ABSA + GlyOH to CPK container and mix

4. Aliquot 50 μL stocks on ice

5. Store at -20°C