Karenliu
| The Liu Lab | Department of Craniofacial Biology | Kings College London |
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Welcome to the Liu Lab!
We are interested in signal transduction in developmental biology.
In any intricate developmental process, the encoded genes are interpreted as myriad intercellular and intracellular interactions, all of which must occur at the right time and place. With this in mind, we are developing new methods to block or alter the activities of individual proteins in a drug-dependent manner. Our goal is to expand the repertoire of molecular tools available to developmental biologists. Rather than screen for small molecule-protein interactions we have chosen to use a directed approach to making chemically sensitive protein alleles, in which we use drug-dependent tags to regulate protein stability and localization.
The biological problem that we are interested in is the development of the neural crest and its derivatives, including the craniofacial skeleton. At present, we are developing chemical tools to study the roles of GSK-3 and Wnt signaling in the neural crest. We are using two model systems, the frog Xenopus laevis and the mouse. Xenopus embryos are abundant and live in an aquatic environment, allowing easy manipulation and drug accessibility; thus, we are using Xenopus to study early patterning and to rapidly test new tools. We then adapt these tools to mammalian systems. In the mouse, we are currently studying the development of the bony skull, using conventional and drug-dependent alleles of GSK-3β.
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Recent updates to the lab wiki
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26 July 2024
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13:51 Elizabeth Suesca talk contribs uploaded File:Fluorometro .jpg | ||||
| N 12:42 | UA Biophysics: Analytical Balance Instructions ESP diffhist +1,106 Elizabeth Suesca talk contribs (Created page with "'''Si no tiene autorización para usarlo por favor comunicarse con biofisica@uniandes.edu.co''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>Uso de la balanza</h2> #La balanza solo debe ser usada para pesar materiales solidos o líquidos en recientes bien cerrados. #Esta balanza no se usará para pesar medios de cultivo celular. #El peso (materi...") | ||||
| 09:58 | UA Biophysics: Lyophilizer ESP diffhist +15 Elizabeth Suesca talk contribs | ||||
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09:18 | UA Biophysics:Protocols 3 changes history +123 [Elizabeth Suesca (3×)] | |||
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08:34 (cur | prev) +1,268 Elizabeth Suesca talk contribs (Created page with "Limpieza laminillas con Solución piraña '''Materiales:''' • Ácido sulfúrico H2SO4 • Peróxido H2O2 al 50% • Isopropanol '''Procedimiento:''' '''Día 1:''' En cabina de extracción con guantes de nitrilo de alto calibre: *En un vaso de 25 ml de vidrio preparar 20 ml de Solución H2SO4: H2O2 al 30% agregando el peróxido al agua (8 ml de agua y 12 ml de peróxido). *En vaso de 250 ml colocar 60 ml de Ácido sulfúrico *Se le agregan los 20 ml del peróxido m...") | |||
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24 July 2024
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