HughesLab:Protocols
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Molecular Biology
- Basic qPCR protocol using the ABI7900HT machine and Taqman probes: File:RNA prep using Trizol.docx
- Arcturus RiboAmp RNA amplification from fly brains: File:RiboAmp1.docx
- Basic RT reaction using ABI Archive cDNA kit: File:RT protocol.docx
- RNA prep using Trizol and Qiagen RNeasy columns: File:TrizolRNAProtocol.v1.pdf
- Protein Extract RIPA Recipe from Xiping Zhang at Esser Lab: File:EsserRIPABuffer.pdf
- New cDNA reverse transcription protocol for WUSTL: File:CDNAprotocol.wustl.pdf
- Human Muscle RNA Extraction from L.R. File:RNA extraction from human muscle.pdf
Cell Culture
- C2C12 Harvest for Protein Lysate: File:Cultured C2C12s to Protein Lysate.pdf
Protein Biology
- Tentative Western Protocol: File:WesternProtocolHughes31July2019.pdf
Mouse Colony Management, Genotyping, etc.
- Mouse tail digestion protocol for genotyping: File:MouseTailingProtocol.pdf
- PCR and Genotyping Protocol: File:HughesGenotypingProtocol.pdf
- EtBr Gel Imaging Settings: File:EtBrGelImagingSettings.pdf
Next-generation sequencing
- Pilot protocol for PCR amplifying fly RNA editing sites: File:Editingseq pilot.docx
- miRNA TruSeq Library Prep: File:MiRNA library prep protocol Illumina.pdf
Fly Behavior and Genetics
- Drosophila virgin collection: File:Drosophila virgin collection.docx
- Vials for DAM analysis: File:Fly food for DAM analysis.docx
- Fly food protocol: File:Fly food.docx
Analytics
- Human temperature record analysis: File:Human Temp Analysis.docx
- RUM (RNA-Seq Unified Mapper) guide: File:RUM guide.pdf
- Basic linux commands: File:Linux commands.pdf
- Willie's Aligning Sheet (26 March 2019): File:ShellBashLinuxAligningSTAR.tips.pdf
Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine