Endy:Screening plasmid/v1.0
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Design
Controls
- <bbpart>I13522</bbpart> - constitutive GFP
- <bbpart>I13521</bbpart> - constitutive RFP
- <bbpart>I13540</bbpart> - inducible GFP
- <bbpart>I13520</bbpart> - inducible RFP
- <bbpart>V1023</bbpart> - negative (CW2553/pJAt8)
Characterization of Empty Screening Plasmid 1.0
- Endy:Screening plasmid/v1.0/Hairpin - characterize effectiveness of the hairpin to stabilize the mRFP1 transcript
- Endy:Screening plasmid/v1.0/RNAseE sites - characterize effectiveness of the RNAseE sites
- Endy:Screening plasmid/v1.0/Growth rates - characterization of growth rates under different induction levels / conditions
- Endy:Screening plasmid/v1.0/Steady state - evaluate expression levels at various time points following induction.
- Endy:Screening plasmid/v1.0/Characterization protocol
To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output).
Measurement of Parts and Devices using Screening Plasmid 1.0
Terminator Characterization via Screening Plasmid 1.0
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/9/93/TermsCorrected.jpg/300px-TermsCorrected.jpg)
Inverter Characterization via Screening Plasmid 1.0
Inverter library screening via Screening Plasmid 1.0
To be added.