- What are the most common things people end up needing to repair when they build their devices (or do everyone else's just work the first time)? (expression level, mRNA stability, protein function, RNA/DNA secondary structure, etc)
- Do people frequently use screens now to repair/build their devices? What sort of screens do they use? How can we incorporate more complicated screening and selection for tuning/constructing our devices?
Screening Plasmid specific
- need to move to lower copy if we want to test 2 inverters in series (o/w get a pretty big growth hit)
- Would like to move to an induction system that doesn't require co-transformation with a plasmid and accommodates more host strains (e.g. pBAD requires native arabinose transport to be knocked out).
Terminator Characterization via Screening Plasmid 1.0
Inverter Characterization via Screening Plasmid 1.0