Biomod/2011/Harvard/HarvarDNAnos:Results

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<html><center><a href='http://openwetware.org/wiki/Biomod/2011/Harvard/HarvarDNAnos'><img src=http://openwetware.org/images/1/1c/Hdnaheader.jpg width=800px></a></center></html>

Home              Mission              Process              Designs              Results              Resources              Team

Results Overview

We have accomplished the following:

  • With our original rectangular box design, we have developed a system that can trap, hold, and release nanocargo.
  • We have opened Han’s sphere in preparation for future nanocargo encapsulation.
  • We are now one step closer to achieving simpler signal amplification, more complex logic gates, and submarine-like drug delivery.

Box Container

Figure 1. A box with enclosed gold under a transmission electron microscope.
  • We designed and folded two robust origami structures: the lid and the barrel. These two structures are the components of our box.
  • We demonstrated that we can close and open the box with high efficiency.
  • We demonstrated that we can attach AuNP cargo to our lids and/or our barrels and then close the box, placing the AuNP cargo within the box.
  • We demonstrated that we can photocleave the AuNP cargo off of our lids, solubilizing the cargo.


Continue reading...

See also: Rectangular Box Design, Rectangular Box Methods

Spherical Container

Figure 2. Open spheres under an atomic force microscope.

With our sphere design, we were able to:

  • Fold and characterize through atomic force microscopy (AFM) the original Han et al. sphere, which we call the "closed" sphere.
  • Fold and characterize through AFM our "open" sphere in which we removed all equator staple strands.
  • Test various lock mechanisms to transition between the closed and open states of the sphere.
  • Analyze the scaffold-staple-lock system to find out why certain lock designs were unsuccessful at closing the sphere.


Continue reading...

See also: Sphere Design, Sphere Methods

Nanoparticles

Figure 3. Evidence of DNA conjugation to gold nanoparticles
  • We synthesized 5nm gold particles and conjugated DNA to their surfaces
  • We conjugated DNA to commercially available 5 nm gold nanoparticles
  • We integrated AuNP-DNA conjugates into our rectangular box design


Continue reading...
See also: Cargo Design

Photo-cleavage

  • We demonstrated photo-cleavage of strands with internal photo-cleavable spacers
  • We outlined a process for the use of photo-cleavable spacers as a means to open our containers and solubilize our cargo


Continue reading...
See also: Cargo Design

Tools and Parts
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