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In order to maintain the utility of our GAIIs, the BioMicro Center has developed methods to run the GA in a lane-wise manner. These methods are not supported by Illumina and we are learning by trial and error. The partial flowcell effort was lead by Michael Gravina in the BioMicro Center.
|Testing lanewise flow||Food coloring was loaded in the GAII using one of the standard 50ml conicals and flowed through a used flowcell either 1 lane at a time or 4 lanes at a time.|
|Testing reclustering of the flowcell||A single flowcell was subjected to 5 cycles of clustering and sequencing over ~2 weeks and evaluated for various quality parameters. PhiX was used as the test sample. Sequencing reagents were replaced for the 4th sequencing cycle.|
We will soon be publishing the scripts we use on the GAII to run partial flowcells. Keep an eye on this space.