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The Covaris E220evolution sonicator is a multitube system developed for high performance sample preparation. The E220evolution enables multi-sample, batch preparation, capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 8 samples in a single batch. The E220 is most appropriate for samples less than 1,000 mg
and volumes less than 10 ml. Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml.
The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.
Protocols for optimizing the sonication time for next generation sequencing can be found on the company's website: http://covarisinc.com/wp-content/uploads/pn_010158.pdf
The manual can be found here: http://covarisinc.com/wp-content/uploads/pn_010025.pdf
Schedule time on the Covaris