BISC209/S13: Use and Care of Micropipets

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Wellesley College-BISC 209 Microbiology -Spring 2013

Use and Care of Micropipets

Pipet use and care: Pipetes are precision instruments—treat them carefully and gently! Ask if you have any questions.

Do not leave your pipetman in places on the bench where they could be knocked off

Set the volume before you put tips on the pipette

Make sure the tips are put on carefully and tightly

Once tips are attached, do not set pipette down or let tip touch anything

Draw liquid up (push plunger down to first stop, put it into the liquid, and slowly raise plunger; make sure to give a slight pause before you lift the tip out of the liquid to ensure that the full and correct volume is drawn up into the tip)

Make sure visually that it is at the expected level and there aren’t any air bubbles

If using a multichannel pipet—make sure all tips are full and the liquid volume is even

Once filled with liquid, make sure you keep the pipette upright to prevent liquids from running back into the pipet shaft

To dispense, move tip to destination tube, push plunger down to first stop, pause, then fully depress the plunger to expel all the liquid; move tip out of liquid before releasing plunger

Eject tip into biowaste container or temporary beaker on the bench top

Practice using plain water until you are comfortable with all of these steps