BISC209/S13:Schedule
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BISC209 S13 Lab Calendar
| Monday | Tuesday | Wednesday | Thursday | Friday |
|---|---|---|---|---|
| Jan.28 Classes begin |
Jan. 29 NO LAB |
Jan. 30 NO LAB |
Jan. 31 | Feb. 1 |
| Feb 4 | Feb. 5 Lab 1 |
Feb. 6 Lab 1 |
Feb. 7 | Feb. 8 |
| Feb. 11 | Feb. 12 Lab 2 |
Feb. 13 Lab 2 |
Feb. 14 | Feb. 15 |
| Feb. 18 Presidents' Day NO CLASSES |
Feb. 19 NO LAB Monday Schedule |
Feb. 20 NO LAB |
Feb. 21 | Feb. 22 |
| Feb. 25 | Feb. 26 Lab 3 |
Feb. 27 Lab 3 |
Feb. 28 | Mar. 1 |
| Mar. 4 | Mar. 5 Lab 4 |
Mar. 6 Lab 4 |
Mar. 7 | Mar. 8 |
| Mar. 11 | Mar. 12 Lab 5 |
Mar. 13 Lab 5 |
Mar. 14 | Mar. 15 |
| Mar. 18 Spring Break |
Mar. 19 Spring Break |
Mar. 20 Spring Break |
Mar. 21 Spring Break |
Mar. 22 Spring Break |
| Mar. 25 | Mar. 26 Lab 6 |
Mar. 27 Lab 6 |
Mar. 28 | Mar. 29 |
| Apr. 1 | Apr. 2 Lab 7 |
Apr. 3 Lab 7 |
Apr. 4 | Apr. 5 |
| Apr. 8 | Apr. 9 Lab 8 |
Apr. 10 Lab 8 |
Apr. 11 | Apr. 12 |
| Apr. 15 Patriots' Day |
Apr. 16 Lab 9 Maldi-TOF Biotyper ID |
Apr. 17 Lab 9 Maldi-TOF Biotyper ID |
Apr. 18 | Apr. 19 |
| Apr. 22 | Apr. 23 No Lab |
Apr. 24 Rhulman NO LAB |
Apr. 25 | Apr. 26 |
| Apr. 29 | Apr. 30 Lab 10 Lab Practical and Final Paper Consultation |
May 1 Lab 10 Lab Practical and Final Paper Consultation |
May 2 | May 3 |
| May. 6 | May 7 NO LAB Tues Lab Paper Due |
May 8 NO Lab Wed Lab Paper Due Last day of classes |
May 9 | May 10 |
Weekly Topics
BISC209 Weekly Lab Topics
| Lab # | LAB DATES__ | IN LAB WORK__________________ | OUTSIDE OF LAB WORK________ | |||
| 1 | Tues. 2/5- Wed. 2/6 |
Introduction to Microbiology Lab Lab Safety |
Visit the greenhouse and make notes on your selected habitat.
| |||
| 2 | Tues. 2/12- Wed. 2/13 |
Finish Plate Count & quantify cultured microbes by culture dependent method; Practice Streaking for Isolation; Make soil extract from dried soil and set up isolation of spore-forming bacteria; Continue enrichment & isolation of selected groups of bacteria | Assess your isolation streaking and continue to isolation streak to pure culture. ON the MORNING OF LAB 3 Collect A NEW SOIL SAMPLE from your group's sampling site. (Materials available for pick up in the lab.) | |||
| 3 | Tues. 2/26- Wed. 2/27 |
Isolation of Culturable Bacteria. Evaluate streaking for isolation. Make another soil extract and serial dilution Start CLPP: Community Level Physiological Profiling: Carbon source utilization; Continue selection & isolation, of desired bacterial groups; |
Collect data from BIOLOG ECO plates;. Check on cultures and continue isolation to pure culture. | |||
| 4 | Tues. 3/5- Wed. 3/6 |
Isolation of Culturable Bacteria: Examine cultures and pick unique isolated colonies of your soil bacteria Community Exoenzyme assessment; CLPP analysis and calculations of carbon source utilization |
Complete isolation to pure culture and set up fresh pure cultures 24-48 hours prior to next lab. | |||
| 5 | Tues. 3/12- Wed. 3/13 |
Culturable Bacteria: Make new cultures from each of your pure cultures of your soil bacteria isolates. Use a pure culture of each isolate to its amplify 16srRNA gene by pcr. Clean up pcr product. Perform agarose gel electrophoresis of pcr product to assess success of amplification. |
Set up fresh isolate cultures 24-48 hours prior to next lab as needed. | |||
| 6 | Tues. 3/26 - Wed. 3/27 |
Isolate Testing: Perform physical characteristics tests: smear slide, Gram stain, confirm Gram stain with selective media, start an antibiotic production test. |
Set up fresh isolate cultures 24-48 hours prior to next lab as needed. | |||
| 7 | Tues. 4/2- Wed. 4/3 |
Isolates Testing: Start motility test and MNM tests; Continue antibiotic production test; Read Gram stain confirmation by selective media, Start Bacterial interactions test. |
Set up fresh isolate cultures 24-48 hours prior to next lab. | |||
| 8 | Tues. 4/9- Wed. 4/10 |
Cont. Isolates testing: Complete and read antibiotic production test, interactions, MNM tests. Special Staining as indicated. |
Set up fresh isolate cultures 24 hours prior to next lab for freezing. | |||
| 9 | Tues. 4/16- Wed. 4/17 |
Maldi-TOF biotyper ID of isolates and freezing Isolates |
Set up fresh isolate cultures if needed. | |||
| 10 | Tues. 4/23- Wed. 4/24 RHULMAN |
No Formal LABS - Freeze isolates if not completed in lab 9 |
| |||
| 11 | Tues. 4/30- Wed. 5/1 |
Lab Practical and Paper Rough Draft |
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| 12 | Tues. 5/7- Wed. 5/8 |
PAPER DUE | Final Paper Due by 5pm uploaded to Sakai AND in hard copy to your instructor's office if she requests it. |
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