Lidstrom:Protocols

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Revision as of 00:20, 14 March 2012 by Janet B. Matsen (talk | contribs) (→General Lab:)
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Back home: Lidstrom

Contents

  • 1 General Lab:
  • 2 DNA/RNA:
    • 2.1 DNA
    • 2.2 RNA
  • 3 Equipment Use

General Lab:

  • Finding Chemicals
  • Autoclave
  • Sterile technique
  • E. Coli basics
  • Preparing Freezer Cell Stocks
  • Pouring Media Plates

DNA/RNA:

DNA

  • Intro: Building with DNA (in progress)
  • Colony PCR
  • Digestion with Restriction Enzymes
  • Diluting Primers
  • Gibson Assembly (In progress)
  • Ligation (in progress)
  • Miniprep (in progress)
  • Oligo Orders (in progress)
  • Overlap Extension PCR (in progress)
  • PCR (in progress)
  • Purifying DNA (in progress)
  • Sequencing with GeneWiz
  • Site-Directed Mutagenesis (in progress)
  • Transformation (in progress)

RNA

Equipment Use

  • Baker Lab LCMS
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  • This page was last edited on 14 March 2012, at 00:20.
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