IGEM:IMPERIAL/2006/project/Oscillator/project browser/Full System/TestingValidation: Difference between revisions

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!height="25pt" width="80pt"|Actual Part
!height="25pt" width="80pt"|Actual Part
!colspan="2"| [[Image:J37036 logo.png]]  
!colspan="2"| [[Image:J37036 part.png]]  
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!height="25pt" width="80pt"|Sub Parts
!height="25pt" width="80pt"|Sub Parts

Revision as of 02:31, 29 October 2006

Super Parts Not applicable
Actual Part
Sub Parts Prey Construct Predator Construct


Motivations

  • To see whether oscillations are produced by measuring fluctuating levels of AHL that match our specifications:
  • Stable oscillations for greater than 10 cycles
  • High signal to noise ratio
  • Control over frequency and amplitude

Enviroment

This system will run in a Chemostat. This pice of equipment will keep the cell populations constant and thus keep the pareameters constant throught the course of the experiment. chemostat page

Protocol

  • A formal protocol has not been written.
  • A suggested method was to aliquot the extracellular medium within the chemostat at certain time points and then measure the [AHL] using the T9002/J37016 assay

Results

  • The predator construct has to be built.