| MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
note
|
|
|
|
Tue Feb 2
|
ANS
|
|
Orientation
|
* Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
|
|
|
| 1
|
1
|
Thur Feb 4
|
JN
|
CB
|
Amplify aptamer-encoding DNA
|
* M1D1 Lecture
* M1D1 Pre-Lab
|
| 1
|
2
|
Tue Feb 9
|
JN
|
CB
|
Purify aptamer-encoding DNA
|
* M1D2 Lecture
* M1D2 Pre-Lab
|
| 1
|
3
|
Thur Feb 11
|
JN
|
CB
|
Prepare RNA by IVT
|
* M1D3 Lecture
* M1D3 Pre-Lab
|
|
|
|
President's Day Feb 15
|
No lab Feb 16 or Feb 17
|
| 1
|
4
|
Thur Feb 18
|
JN
|
CB
|
Purify RNA and run affinity column
|
* M1D4 Lecture
* M1D4 Pre-Lab
|
| 1
|
5
|
Tue Feb 23
|
JN
|
CB
|
RNA to DNA by RT-PCR
|
* M1D5 Lecture
* M1D5 Pre-Lab
|
| 1
|
6
|
Thur Feb 25
|
JN
|
CB
|
Post-selection IVT and journal club
|
* M1D6 Lecture
* M1D6 Pre-Lab
|
| 1
|
7
|
Tue Mar 2
|
JN
|
CB
|
Aptamer binding assay
|
* M1D7 Lecture
* M1D7 Pre-Lab
|
| 1
|
8
|
Thur Mar 4
|
JN
|
CB
|
Journal club
|
* M1D8 Lecture
|
|
|
| 2
|
1
|
Tue Mar 9
|
AJ
|
XY
|
Start-up protein engineering
|
Module 1 Draft Due
* M2D1 Lecture
* M2D1 Pre-Lab
|
| 2
|
2
|
Thur Mar 11
|
AJ
|
XY
|
Site-directed mutagenesis
|
* M2D2 Lecture
* M2D2 Pre-Lab
|
| 2
|
3
|
Tue Mar 16
|
AJ
|
XY
|
Bacterial amplification of DNA
|
* M2D3 Lecture
* M2D3 Pre-Lab
|
| 2
|
4
|
Thur Mar 18
|
AJ
|
XY
|
Prepare expression system
|
* M2D4 Lecture
* M2D4 Pre-Lab
|
|
|
|
Spring Break Mar 22-26
|
No lab all week
|
| 2
|
5
|
Tue Mar 30
|
AJ
|
XY
|
Induce protein and evaluate DNA
|
* M2D5 Lecture
* M2D5 Pre-Lab
|
| 2
|
6
|
Thur Apr 1
|
AJ
|
XY
|
Characterize protein expression
|
Module 1 Final Report Due
* M2D6 Lecture
* M2D6 Pre-Lab
|
| 2
|
7
|
Tue Apr 6
|
AJ
|
XY
|
Assay protein behavior
|
* M2D7 Lecture
|
| 2
|
8
|
Thur Apr 8
|
AJ
|
XY
|
Data analysis
|
* M2D8 Lecture
|
|
|
| 3
|
1
|
Tues Apr 13
|
ANS
|
NC
|
Start-up biomaterials engineering
|
Module 2 Draft Due
* M3D1 Lecture
* M3D1 Pre-Lab
|
| 3
|
2
|
Thur Apr 15
|
ANS
|
NC
|
Initiate cell culture
|
* M3D2 Lecture
|
|
|
|
Patriot's Day April 19-20
|
No lab Apr 20 or Apr 21
|
| 3
|
3
|
Thur Apr 22
|
ANS
|
NC
|
Testing cell viability
|
* M3D3 Lecture
* M3D3 Pre-Lab
|
| 3
|
4
|
Tue Apr 27
|
ANS
|
NC
|
Preparing cells for analysis
|
* M3D4 Lecture
* M3D4 Pre-Lab
|
| 3
|
5
|
Thur Apr 29
|
ANS
|
NC
|
Transcript-level analysis
|
* M3D5 Lecture
* M3D5 Pre-Lab
|
| 3
|
6
|
Tue May 4
|
ANS
|
NC
|
Protein-level analysis
|
Module 2 Final Report Due
* M3D6 Lecture
* M3D6 Pre-Lab
|
| 3
|
7
|
Thur May 6
|
ANS
|
NC
|
Wrap-up analysis
|
Module 3 Report Due
*Atissa's Lecture
* M3D7 Pre-Lab
|
| 3
|
8
|
Tue May 11
|
ANS
|
NC
|
Student presentations
|
|
|
|
|
Thur May 13
|
|
|
No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
|
|
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