20.109(F07):Module 3

From OpenWetWare
Jump to navigationJump to search

20.109(F07): Laboratory Fundamentals of Biological Engineering

Home        People        Schedule Fall 2007        Assignments        Lab Basics        OWW Basics       
Genome Engineering        Expression Engineering        Biomaterials Engineering              

Module 3

Instructors: Angela Belcher, Natalie Kuldell and Agi Stachowiak

TA:Robbie Barbero

“Invention” is a wonderful word, derived from words meaning “scheme” and “a finding out.” Inventors draw on materials provided by the natural world, refining and combining them in insightful ways, to make something useful. In this experimental module we will invent materials by manipulating biological systems, namely the bacteriophage M13. We will use a very slightly modified phage to build Iridium nanowires that we’ll visualize on the transmission electron microscope. Then we'll let the phage themselves do the building, making an electrochromic device that's both fun and potentially useful. Drawing on the rich stockroom of biological elements and a good but incomplete understanding of their behavior, we’ll hope to invent some novel materials with real-world applications.

TEM of M13E4 after CoCl2/NaBH4 treatment, image by Natalie Kuldell, Anthony Garratt-Reed and KiTae Nam

Module 3 Day 1: growth of phage materials
Module 3 Day 2: making nanowires
Module 3 Day 3: TEM
Module 3 Day 4: Phage by design
Module 3 Day 5: Phage by design, pt 2
Module 3 Day 6: ECD assembly
Module 3 Day 7: oral presentations
direct link to experimental variations page

TA notes, mod 3