DNA
From OpenWetWare
Contents |
General pages
- Category:Reagents
- Category:User DNA unzipping
- ColE1
- DNA stability
- Escherichia coli/Vectors
- Image:Shotgun DNA unzipping.JPG
- Image:Xia, Gamble et al nanoporous nanochannels.gif
- Koch Lab:Publications
- Making construction plasmid
- Nucleic acid structure
- P15A
- PSC101
- Topoisomerase I mediated TA cloning
- Vectors
Protocols
General
- 'Round-the-horn site-directed mutagenesis
- Affymetrix DNA labelling for gene expression arrays
- Agarose gel electrophoresis
- Anneal Extend PCR Protocol - for linking 2 pieces of DNA
- Annealing and primer extension
- Annealing primers
- Arabidopsis gDNA isolation
- Assembly pcr
- BLAST
- Bioanalyzer
- Cell and tissue lysis hub
- Centrifugal filtration/Nucleic acids
- Colony PCR
- DNA Precipitation
- DNA Synthesis from Oligos
- DNA extraction - Salting Out
- DNA extraction from tissue
- DNA ligation
- Denaturing acrylamide gel purification of nucleic acids
- Designing primers
- Electrophoretic mobility shift assay
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Engineering BioBrick vectors from BioBrick parts/DNA ligation
- Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
- Engineering BioBrick vectors from BioBrick parts/Restriction digest
- Ethanol precipitation of nucleic acids
- Ethanol precipitation of small DNA fragments
- Filamentous fungi genomic DNA isolation
- Image:Low tech flow cell and wicks.JPG
- Image:Low tech flow cell changing buffer with wick.JPG
- Image:Low tech flow cell.JPG
- Image:Unzipping construct.jpg
- Library Generation
- Maxiprep of plasmid DNA from E. coli
- Miniprep
- Miniprep/GET buffer
- Miniprep/Kit-free high-throughput protocol
- Miniprep/Qiagen kit
- Mouse tissue lysis for genotyping
- One step 'miniprep' method for the isolation of plasmid DNA
- OpenWetWare:New visitor blurb
- PCR
- PCR Overlap Extension
- PCR techniques
- PNK Treatment of DNA Ends
- Phenol/chloroform extraction
- Phosphatase treatment of linearized vector
- Plasmapper
- Protocol Size selective DNA precipitation by PEG/MgCl2
- Purification of DNA
- Q-PCR
- QRT-PCR
- Quantitating nucleic acids
- Real-time PCR
- Restriction digest
- Resuspension of primers
- Sequencing DNA
- Site-directed mutagenesis
- Streptavidin purification of DNA fragments
- TempliPhi
- Yeast Colony PCR
- Yeast DNA Prep
Lab-specific protocols
- "Core Sample" PCR: Amplifying DNA from agarose gels
- Alm:Agarose gel electrophoresis
- Duffy:LIC Cloning
- Endy:Double stranding oligo libraries
- Endy:Templiphi
- Etchevers:ChIP francais
- Freimoser:Protocols:FastYeastTransformation
- Jamesh008:consensus DNA ligation protocol
- Knight:Agarose gel electrophoresis
- Knight:Annealing and primer extension with Klenow polymerase
- Knight:Annealing and primer extension with Taq polymerase
- Knight:Colony PCR
- Knight:DNA ligation using NEB Quick Ligation Kit
- Knight:DNA ligation using T4 DNA ligase
- Knight:Electrophoretic mobility shift assay
- Knight:Maxiprep
- Knight:Micropure EZ and Microcon purification
- Knight:Miniprep low copy plasmids
- Knight:PhoenIX Maxiprep Kit
- Knight:Reconstituting primers
- Knight:Restriction Digest
- Knight:Sequencing DNA
- Knight:Site-directed mutagenesis/Multi site
- Knight:Site-directed mutagenesis/Single site
- Knight:TOPO TA cloning
- Knight:TempliPhi
- Koch Lab:Protocols/Dig-bio PCR
- Koch Lab:Protocols/Dig-bio PCR/pRL574 4411 PCR
- Koch Lab:Protocols/Fork unzipping constructs
- Koch Lab:Protocols/Loading rate clamp
- Koch Lab:Protocols/Microsphere-DNA tethering
- Koch Lab:Protocols/Oligonucleotide Annealing/Duplexes
- Koch Lab:Protocols/Oligonucleotide Annealing/Hairpins
- Koch Lab:Protocols/Unzipping constructs
- Koch Lab:Protocols/Unzipping constructs/17mer
- Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex
- Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment
- Koch Lab:Protocols/Unzipping constructs/17mer/Downstream unzipping segment
- Lory:Quick transformant screen
- MIT:Sequencing BioBrick DNA
- NanoBio: PCR
- Sauer:Stitching Genes by PCR
- Seth:Quick Transformant Screen
- Silver: PCR
- Streptomyces:Protocols/DNA Digest
Materials
- Cell and tissue lysis hub
- DNA ligase
- DNA polymerase
- Pfu
- Phusion
- Reverse Transcriptase
- T4 DNA Polymerase
- Terminal Transferase
- Thermostable DNA ligase
Resources
See Web resources#DNA and Web resources#DNA 2
