Muratore:Protocols/Digest/SapI

From OpenWetWare

Jump to: navigation, search
SapI digest protocol
Tube sterile H2O 10X NEB4 DNA SapI (2 kU/mL)
plasmid 30 μL 5 μL 10 μL of NheI digest 5μL
insert 30 μL 5 μL 10 μL of NheI digest 5μL
  1. 2h @ 37°C
  2. spin-column purify
Personal tools