Thermostable DNA ligase

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[edit] Purpose

  • Thermostable DNA ligases can be added to a PCR reaction to ligate extended oligos. This is used for some mutagenesis methods.

[edit] Procurement

  • Examples for thermostable DNA ligases are Taq ligase NEB and the Ampligase Epicentre.

[edit] Use

  • If you want to ligate extended oligos in a PCR reaction it is important to have them phosphorilated at the 5'-end. Oligos will only be ligated when they are assembled on a compementary strand.
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