Muratore:Protocols/Digest/NheI

NheI digest protocol

Tube	sterile H2O	10X NEB2	100X BSA	DNA	NheI (10 kU/mL)
plasmid	34.5 μL	5 μL	0.5 μL	5 μL of 100-500 μg/mL	5μL
insert	24.5 μL	5 μL	0.5 μL	15 μL from purified PCR reaction	5μL

1. 2h @ 37°C
2. 20' @ 65°C (heat kill)