User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/18

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Summary

  • Short summary

Method

  • Students T-test on all ELISA's done so far
    • Ht31/Ht31P done separately from Ht31/S0
  • Student T-test, 2-tailed, α = 0.025

Results

T.TEST S0 & Ht31
S0 p = CSE (S0) p = CSE (Ht31) p =
CTR vs CSE 0,1078 vs CTR (Ht31) 0,120 vs CTR (S0) 0,003 <0.05
CTR vs CSE+8p 0,03 <0.05 vs CSE+8P (Ht31) 0,176 vs CSE+8P (S0) 0,023 <0.05
CTR vs CSE+6Bnz 0,065 - vs CSE+6Bnz (Ht31) 0,230 vs CSE+6Bnz (S0) 0,003 <0.05
CSE vs CSE+8p 0,642 -
CSE vs CSE+6Bnz 0,158 -
CSE+8p vs CSE+6Bnz 0,070 CSE+8p (S0) CSE+8p (Ht31)
Ht31 vs CTR (Ht31) 0,043 <0.05 vs CTR (S0) 0,006 <0.05
CTR vs CSE 0,003 <0.05 vs CSE+6Bnz (Ht31) 0,273 vs CSE+6Bnz (S0) 0,008 <0.05
CTR vs CSE+8p 0,007 <0.05
CTR vs CSE+6Bnz 0,058 -
CSE vs CSE+8p 0,751 - CSE+6Bnz (S0) CSE+6Bnz (Ht31)
CSE vs CSE+6Bnz 0,005 vs CTR (Ht31) 0,162 vs CTR (S0) 0,040 <0.05
CSE+8p vs CSE+6Bnz 0,012 <0.05
S0 vs Ht31
CTR 0,426
CSE 0,219 -
CSE+8p 0,032 <0.05
CSE+6Bnz 0,157 -
DATA
(IL-8 Conc. pg/mL)
S0
CTR 33,76791033 10,85496714 10,95450842 64,14649613 10,85496714
CSE 239,8091037 106,32513 825,6543713 235,7278169 106,32513
CSE+8P 414,0943476 95,56721794 350,9905481 192,1552867 95,56721794
CSE+6Bnz 128,2774353 39,33258922 56,44477041 86,85648194 39,33258922
Ht31
CTR 37,97064287 21,42525141 51,83692369 70,85919552 11,16153545
CSE 503,5237738 273,5458268 734,3264253 462,3291814 590,7760679
CSE+8P 574,5179107 259,3276989 672,8539828 416,2929523 850,939156
CSE+6Bnz 90,90877671 83,34906579 104,8848809 124,7685078 289,1702769

Notes

  • Due to the low cell viability caused by Ht31P the results obtained from Ht31P and the Ht31 in combination with Ht31P gave no good results
  • Because Ht31P caused cell death the ratio of Alamar blue of Ht31 was high in comparison to S0 yielding too low IL-8 values
    • IL8 value corrected for Alamar blue = )IL8 value/percentage of cell viability compared to CTR Ht31P' × 100

Conclusion

  • CSE+S0 has a too large Standard Deviation
    • A higher n is required
    • More experiments will be started


  • PKA is not significantly inhibited by Ht31
    • Increase Ht31
    • Perform VASP in increasing Ht31 concentrations


  • Epac does not effect IL-8 production


  • PKA decreases IL-8 production

Related entries

Run 1: Ht31/Ht31P D9/D12

Run 2: Ht31 Dose/Effect Curve & S0 D9/D12

Run 3: Ht31/S0