Summary
Method
- Students T-test on all ELISA's done so far
- Ht31/Ht31P done separately from Ht31/S0
- Student T-test, 2-tailed, α = 0.025
Results
T.TEST
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S0 & Ht31
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S0
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p =
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CSE (S0)
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p = |
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CSE (Ht31)
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p = |
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CTR vs CSE
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0,1078 |
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vs CTR (Ht31) |
0,120 |
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vs CTR (S0) |
0,003 |
<0.05
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CTR vs CSE+8p
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0,03 |
<0.05
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vs CSE+8P (Ht31)
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0,176 |
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vs CSE+8P (S0)
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0,023 |
<0.05
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CTR vs CSE+6Bnz
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0,065 |
-
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vs CSE+6Bnz (Ht31)
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0,230 |
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vs CSE+6Bnz (S0)
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0,003 |
<0.05
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CSE vs CSE+8p
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0,642 |
- |
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CSE vs CSE+6Bnz
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0,158 |
- |
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CSE+8p vs CSE+6Bnz
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0,070 |
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CSE+8p (S0) |
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CSE+8p (Ht31) |
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Ht31
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vs CTR (Ht31)
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0,043
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<0.05
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vs CTR (S0)
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0,006
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<0.05
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CTR vs CSE
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0,003
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<0.05
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vs CSE+6Bnz (Ht31)
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0,273 |
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vs CSE+6Bnz (S0)
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0,008 |
<0.05
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CTR vs CSE+8p
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0,007 |
<0.05 |
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CTR vs CSE+6Bnz
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0,058 |
- |
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CSE vs CSE+8p
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0,751 |
- |
CSE+6Bnz (S0) |
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CSE+6Bnz (Ht31) |
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CSE vs CSE+6Bnz
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0,005 |
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vs CTR (Ht31)
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0,162 |
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vs CTR (S0)
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0,040 |
<0.05
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CSE+8p vs CSE+6Bnz
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0,012 |
<0.05 |
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S0 vs Ht31
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CTR
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0,426
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CSE
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0,219 |
- |
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CSE+8p
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0,032 |
<0.05 |
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CSE+6Bnz
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0,157 |
- |
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DATA (IL-8 Conc. pg/mL)
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S0
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CTR
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33,76791033 |
10,85496714 |
10,95450842 |
64,14649613 |
10,85496714
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CSE
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239,8091037 |
106,32513 |
825,6543713 |
235,7278169 |
106,32513
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CSE+8P
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414,0943476 |
95,56721794 |
350,9905481 |
192,1552867 |
95,56721794
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CSE+6Bnz
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128,2774353 |
39,33258922 |
56,44477041 |
86,85648194 |
39,33258922
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Ht31
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CTR
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37,97064287 |
21,42525141 |
51,83692369 |
70,85919552 |
11,16153545
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CSE
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503,5237738 |
273,5458268 |
734,3264253 |
462,3291814 |
590,7760679
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CSE+8P
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574,5179107 |
259,3276989 |
672,8539828 |
416,2929523 |
850,939156
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CSE+6Bnz
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90,90877671 |
83,34906579 |
104,8848809 |
124,7685078 |
289,1702769
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Notes
- Due to the low cell viability caused by Ht31P the results obtained from Ht31P and the Ht31 in combination with Ht31P gave no good results
- Because Ht31P caused cell death the ratio of Alamar blue of Ht31 was high in comparison to S0 yielding too low IL-8 values
- IL8 value corrected for Alamar blue = )IL8 value/percentage of cell viability compared to CTR Ht31P' × 100
Conclusion
- CSE+S0 has a too large Standard Deviation
- A higher n is required
- More experiments will be started
- PKA is not significantly inhibited by Ht31
- Increase Ht31
- Perform VASP in increasing Ht31 concentrations
- Epac does not effect IL-8 production
- PKA decreases IL-8 production
Related entries
Run 1: Ht31/Ht31P D9/D12
Run 2: Ht31 Dose/Effect Curve & S0 D9/D12
Run 3: Ht31/S0
- Counting cells
- Putting to S0
- Stimulating cells
- Ending stimulation & Viability
- ELISA
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