User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/26

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Owwnotebook icon.pngELISA & Counting splitting cells <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Short summary

Materials & Methods

Materials

Method

ELISA

Sample dilution ELISA (Dilution buffer (μL)/Sample (μL))
1 2 3 4 5 6
A 100/100 100/100 100/100 100/100 100/100 100/100
B 150/50 150/50 150/50 175/25 175/25 175/25
C 150/50 150/50 150/50 175/25 175/25 175/25
D 150/50 150/50 150/50 175/25 175/25 175/25

Counting and splitting cells

Results

  • HT4 cells
    • 286
    • 249
    • 203
    • 193
  • D12 hTERT cells (15.75×104 cells/mL)
    • 20
    • 12
    • 15
    • 16
    • S+ 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
  • D12 P28 of Mark hTERT cells (75.5×104 cells/mL)
    • 92
    • 67
    • 83
    • 60
    • 0.43 mL cells in 23 mL for 24-wells, 3.97 mL in 15 mL for 6 wells
  • D9 (53.5×104 cells/mL)
    • 52
    • 55
    • 64
    • 58
    • 0.61 mL in 13 mL and 5.35 mL in 15 mL
  • ELISA SEE DOSE EFFECT CURVE

Conclusion

  • DONE Ow..

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Run 2: Ht31 Dose/Effect Curve & S0 D9/D12

Same action

Attachment

<html> <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Excel Dose Effect curve D9
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  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Excel Dose Effect curve D12