User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/16
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Stimulation hTERT cells | Main project page Previous entry Next entry | |||||||||||||||||||||||||||||||||||
SummaryTo look at the effect of Protein kinase A (PKA) and Epac on the IL-8 production by airway smooth muscle cells (ASMC's) induced by cigarette smoke extract (CSE), 8-pcpt-2'-o-me-camp (8-pcpt) and 6-Benz-cAMP (6-Benz) are used to activate Epac and PKA respectively. To illustrate the influence of A kinase Anchoring Proteins (AKAP's) an allround inhibitor of PKA (subunit RII) binding to AKAP's HT31 is used. An inactive peptide HT31P is used as an control. The purpose of the described method is to stimulate the cells with the substances to prepare for future experiments (e.g. ELISA, Western blot) Materials & MethodsMaterials
Stock solutions
Method
CTR = control, CSE = Cigarette Smoke extract, 8-pcpt = 8-pcpt-2'-o-me-camp Related entriesRun 1: Ht31/Ht31P D9/D12 |