User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/27

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Ocean Optics Round 3 pH 6 BSA Solution BSA Ocean Optics Solution pH 6 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations 160927 [BSA]= 52.6 uM [Au]= 4.27 mM [HCl]= 1mM Volumes for making the solution: 3 uL HCl 294.6 uL BSA 292.7 uL Au 2409.5 uL HOH Results The chart above shows a dramatic increase in maximum wavelength from about ~450 to ~550 at about ~3200s. The chart above shows a slight decrease in max absorbance until about 1200 sec. at which point it dramatically increases for the rest of the run. The chart above shows a slight decrease in A(535) until about 1200 sec. at which point it dramatically increases for the rest of the run. This is similar to the maximum absorbance over time chart as expected.