User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/10/04

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UV Vis Silver Round 1

pH 4 - 12, Fructose 0 mM, 0.0625 mM

Solutions

  • prepared solutions with HCl, which made our samples ph 4 - 6 useless (AgCl forms and precipitates)

we discarded pH 4 - 6 solutions and only measured absorbance for pH 7 - 12. Observations: all except pH 12 show precipitates. only pH 12 is a homogeneous, slightly orange solution.


volumes and concentrations used:

Fructose = 0.0 mM

Screenshot 2016-10-04 at 1.14.47 PM.png

Fructose = 0.0625 mM

Screenshot 2016-10-04 at 1.17.42 PM.png

we prepared pH 4 - 6 again, with HNO3

Screenshot 2016-10-04 at 1.20.38 PM.png

Screenshot 2016-10-04 at 1.21.26 PM.png


The above screen - shotted spreadsheet can be found in the Dropbox under "AG Stock Solution Calculation Template"

We finished the UV Vis on Wednesday.

Results

0Fructose October 4th A530.PNG

The above chart shows the UV-Vis spectra for [Fructose]=0 mM, [Ag]=0.25 mM. Nanoparticles were only formed for pH 4, 11, and 12. All other pH solutions formed a cream colored precipitate, and did not appear to form nanoparticles from the spectra.


0.0625Fructose October4thspectrum.PNG

The above chart shows the UV-Vis spectra for [Fructose]=0.0625 mM, [Ag]=0.25 mM. As with 0 mM fructose, nanoparticles were only formed for pH 4, 11, and 12. All other pH solutions formed a cream colored precipitate, and did not appear to form nanoparticles from the spectra.


A530 0 fructose redo redo ok.PNG

The absorbance at 530 nm is shown above for [Fructose]=0 mM, [Ag]=0.25 mM. As expected, solutions that did not form nanoparticles have an absorbance of ~0.


0.0625Fructose October4thA530.PNG

The absorbance at 530 nm is shown above for [Fructose]=0.0625 mM, [Ag]=0.25 mM. As expected, solutions that did not form nanoparticles have an absorbance of ~0.