User:Raquel Lara/Notebook/481-2018/2018/09/05

Biomaterials Design Lab

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Objective Make measurement samples from the new stocks of malachite green prepared Measure UV-Vis and fluorescence Description Procedure for stocks and fluorescence samples prepared by Dr.Hartings here Data Stock solutions made by the group G: 0.3ppm, 1ppm, 3ppm, and 6ppm 0.30885 ppm (0.52711 g of 29.3 ppm Malachite green stock, water added to a total mass of 50.0 g) 1.00500 ppm (1.71704 g of 29.3 ppm Malachite green stock, water added to a total mass of 50.049 g) 3 ppm (5.1194 g of 29.3 ppm Malachite green stock, water added to a total mass of 50 g) 6 ppm (1.023 g of 29.3 ppm Malachite green stock, water added to a total mass of 10 g) UV-Vis spectra for malachite green solutions Graph of Concentration vs. Absorbance of UV-Vis spectra for malachite green solutions Fluorescence of Rhodamine stock solutions Area under curve (Integration) TBD The fluorescence standard curve of Rhodamine solution Notes We measured UV-Vis, followed by fluorescence our concentrations(0.3ppm and 1ppm) were too dilute and now signal was picked up by fluorescence We then made two more solutions(3ppm and 6ppm) of higher concentrations and took fluorescence of them, but again, no signal was picked up For the 6ppm we ran out of 50 mL falcon tubes and used 15 mL ones instead and calculations were adjusted to make 10 g of the solution instead of 50. Dr. Hartings then prepared Rhodamine samples that were known to fluoresce Parameters Used: Start(nm): 565 End(nm): 700 Excitation(nm): 555 Ex slit(nm):5 Em slit(nm): 50 Scan Speed(nm/min): 200