User:Raquel Lara/Notebook/481-2018/2018/08/31

Biomaterials Design Lab

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Objective Measure UV-Vis spectrum of different malachite green solutions Make 2 new solutions (using the available stock solutions) and to measure their absorption spectrum As a group, plot the data and determine extinction coefficients. Description Stock solutions Prepared by Dr.Harting: 4.78 ppm (3.79083 g of 63.1 ppm Malachite green stock, water added to a total mass of 50.0521 g) 2.59 ppm (4.75639 g of 26.8 ppm Malachite green stock, water added to a total mass of 49.2598 g) 1.03 ppm (1.86080 g of 26.8 ppm Malachite green stock, water added to a total mass of 48.6527 g) 0.637 ppm (1.1903 g of 26.8 ppm Malachite green stock, water added to a total mass of 50.0786 g) Data Stock solutions prepared by the group G: 0.1ppm & 6ppm 0.100 ppm (7.8702 g of 0.637 ppm Malachite green stock, water added to a total mass of 50.08441 g) 6.01 ppm (4.7617 g of 63.1 ppm Malachite green stock, water added to a total mass of 50.02011 g) 1. UV-Vis spectra for malachite green solutions of the whole class 2. UV-Vis spectra for malachite green solutions taken by group G 3. Graph of Concentration vs. Absorbance for group G UV-Vis results Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.