User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2018/09/05

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Objective The students will make measurement samples from the stock of malachite green that I made yesterday. We'll measure UV-Vis and fluorescence. Description Add experimental record here. Include what, how, and why... Data Rhodamine samples Initial Stock 7.24 mg of Rhodamine B dissolved in methanol and transferred to sample container final mass of 3.3205 g water added to 14.39510 g 502 ppm Sample 1 23.67 mg of Rhodamine stock water added to 15.93720 g 746 ppb Sample 2 10.32 mg of Rhodamine stock water added to 15.61280 g 332 ppb Sample 3 8.26 mg of Rhodamine stock water added to 14.43720 g 287 ppb Sample 4 4.62 mg of Rhodamine stock water added to 14.10721 g 164 ppb Sample 5 2.11 mg of Rhodamine stock water added to 15.94823 g 66.4 ppb Fluorescence measurement parameters Excitation wavelength: 555 nm Emission wavelength start: 565 nm Emission wavelength end: 700 nm Excitation slit width: 5.0 nm Emission slit width: 5.0 nm Scan speed: 200 nm/min Notes Malachite green was not fluorescent, so I made samples of Rhodamine B for fluorescence measurements. The students measured the UV Vis of the malachite green and rhodamine samples and the fluorescence of the rhodamine samples. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. Student Pages RG 2018/09/05 [Alex Lutz 2018/09/05] User:Raquel Lara/Notebook/481-2018/2018/09/05