User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/03/06

Au/BSA Mole Ratios

Main project page Previous entry      Next entry

Objective Experimental section for solution effects of refolding buffer: https://docs.google.com/document/d/1W0ynzs6K51j4vTJHovB_dpGgD1pen7H2dKGgEI1H0Mc/edit To determine the effect of the mole ratio of Au/BSA on AuNP synthesis. Description Effect of Au/BSA Ratio Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4: 90 Au/BSA 110 Au/BSA 130 Au/BSA 150 Au/BSA Dilute to 1 mL with water. Place in oven at 80°C. Leave solutions in oven for 3.5 hr. Effect of Tris Buffer on Protein Fibers Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12. Take UV-vis absorption data. Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12. Data The following graph shows the data obtained over the past 3 weeks for the effect of tris on a fiber solution over time. Notes After working on the BSA calibration curve last week, we noticed that this data collected weekly has begun to show the BSA peak around 280 nm.