User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/02/28
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BSA Calibration Curve
Fibers with Tris Over Time
The following graph shows the BSA spectra of our stock solutions and two dilutions. This data was used to create the calibration curve that follows.
The following graph shows the effect of AuNP fibers with tris over time. The second graph is a zoom-in at the wavelength of interest.
We determined that the liquid in a fiber solution has unreacted gold as well as BSA. The gold UV/Vis peak interferes with the BSA peak at 280nm, therefore, we need a way of determining the amount of gold in solution as well. We plan to use AA Spectroscopy when the instrument is ready.