User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/13

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08/13/10

  • ✓ Order oligos: sequencing primers for pActin5C (based on sequence from Kent Nybakken)



Order oligos
> Received full sequence of "pActin5C" plasmid from Kent Nybakken
> Order oligos to sequence across multiple cloning sites

  1. pA5Cseq f3; 5'-CACTACCGTTTGAGTTCTTGTG
  2. pA5Cseq f4; 5'-CTTGTGCTGTGTGGATACTCC
  3. pA5Cseq r3; 5'-GCTGGAAGGTGGACAGCGAAGC
  4. pA5Cseq r4; 5'-GCGAAGCCAGGATGGAGCCAC