User:Karmella Haynes/Notebook/BioBrick cloning

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Project Description

Creation of parts & assembly of constructs for all projects

  • Polycomb Project
    • Test Gal4-ATF's: built ✓, expression test ✓, functional test ✓
    • Polycomb-ATF's (with myc tag): built ✓, stable lines ✓
    • Histone methylation reporters: built ✓
    • Neo translation induction test constructs: built ✓, functional test ✓ (done, but uncertain outcome)
    • Gal4-PEST-EED neo-inducible expression constructs: built ✓, functional test (failed)
    • Full-length histone methylation sensors (+/- DPRE, constitutive Gal4-EED): built ✓, expression test (in progress)
    • Polycomb-ATF no-VP64 controls: built ✓, stable lines ✓
    • ES cell expression constructs: cloning into pSB31 in progress
  • miR Sensor Project
    • RFP reporter/ repressors: built ✓, expression test ✓
    • CFP reporters: built ✓, expression test ✓
    • 9 pair-wise combos of RFP/CFP: built ✓, expression test ✓
    • 0, 5, 10x UAS site Target constructs: Francesca
    • miR-34 Repressor constructs: Francesca
    • New pcDNA3.1+/ΔCMV,hygro & pcDNA3.1+/ΔCMV,zeo vectors: Andrew
    • miR-34 sensor tests: Daniel


  • Fly miR Sensor Project
    • Basic parts: in progress
    • Transient transfections of BioBricked fluors: pending

Notes

  • This project is: perpetual
  • My status: stilllllll cloning.


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