User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/12

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08/12/10

  • ✓ Order oligo: sequencing primer for pAct5C



Order oligo

  1. pAct5C f1; 5'-GCATGCGTATCGATTGATAAG
  2. pAct5C f2; 5'-CACACATTCAAGCATACAAGC

--> pAct5C r1 worked, but did not read across microMCS. Start from scratch and sequence walk original pAct5C plasmid