User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/25
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High Stability Plasmid Development
Double digestions (DD) were inactivated for twenty minutes at 65°C, as indicated by the enzyme manuals.
Both reactions and their controls were loaded on the same gel:
I prepared two ligation reactions, with plasmid-insert mass concentration ratios of 1:5 and 3:3.
Fibo prepared reactions of his own as well.