User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/26

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Ligation reaction

Ligation reaction of the double digestion (EcoRI, PstI) of the PCR-obtained B0015, and the DD (EcoRI, PstI) of the plasmid pSB1T3.

Ratio 5:0.5 6.5:0.5
DD B0015-PCR E,P 5μL 6.5μL
DD pSB1T3 E,P 0.5μL 0.5μL
Buffer 10x 1μL 1μL
T4 ligasa 1μL 1μL
Agua 2μL 2μL