User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/23
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High Stability Plasmid Development
Today I cleaned the repC region PCR that Fibo performed with Mac. It was diluted finally to 60μL.
The repC region it was ampliffied with oligonucleotides containing the standard preffix and suffix. To assemble it with the plasmid containing a transcriptional terminator pSB1AK3, placing the terminator after the part, I digested with the enzymes EcoRI and SpeI: