User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/24

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High Stability Plasmid Development


  • pSB1AK3/B0015 digestion

PCR digestion

This is the second digestion reaction to link repC with the translational terminator B0015. The standard plasmid containing B0015 was digested with EcoRI-HF and XbaI:

pSB1AK3/B0015 17μL
Buffer 4 5μL
EcoRI-HF 1μL
XbaI 1μL
H2O mQ 25μL
Total 50μL

Inactivations will be done tomorrow.