Stanford/BIOE44:Module 2

Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members	Part	Status	Volume	Concentration
Chris and Karina	pSB2k3 (digested (E and P), extracted, purified)	Ready for Ligation	25 uL	80 ng/uL
Daniel and Aaditya	arsR insert	Digested at E and S	25 uL	36.8 ng/uL
	arsR insert	Digested at X and S, extracted from gel	2 gel bands	(hasn't been purified yet)
Travis and Scott	none
Frank, Claire, and Sanjay	pSB2k3 vector (with K274111 insert)	undigested	a substantial amount	203 ng/ul
	pSB2K3 vector (low copy vector and red-orange insert)	digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet	25uL each	273 ng/uL
	color 004 light green insert	digested X,P and running in gels	~15uL	110 ng/uL
	arsR insert	digested E,S and running in gels	~15 uL	133 ng/uL
	color 004 light green insert	digested X,P and not running in gels yet	40 uL	110 ng/uL
	arsR	digested E,S and not running in gels yet	40 uL	133 ng/uL
Sunny, Alex	color 002 purple insert	digested X,P, gel extracted		0 ng/uL (no DNA retrieved from gels)
	arsR insert	digested E,S, gel extracted		0 ng/uL (no DNA retrieved from gels)
	pSB2K3 vector (with K274111 insert)	undigested	48 uL	8.8 ng/ul
	S03511 vector (low-medium copy #, inducible)	undigested	0uL	41.2 ng/uL
	J13023-1 vector (low-medium copy #, inducible)	undigested	27uL	97.2 ng/uL
	J13023-2 vector (low-medium copy #, inducible)	undigested	27uL	93.7 ng/uL
	J13002-1 vector (low-medium copy #, constitutive)	undigested	28uL	103.9 ng/uL
	J13002-2 vector (low-medium copy #, constitutive)	undigested	29uL	109.1 ng/uL
Kosh & Isis	Purple Color generator	cut at XbaI & PstI, gel extracted (ready for ligation)	~5uL
	Dark Green Color generator	cut at XbaI & PstI, gel extracted (ready for ligation)	~5uL
	Light Green Color generator	cut at XbaI & PstI, gel extracted (ready for ligation)	~5uL