2,216
edits
BISC219/F12: RNAi Lab 7: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Colony PCR
| Line 68: | Line 68: | ||
<br> | <br> | ||
<br> | <br> | ||
'''Primer Sequence:''' specific to T7 RNA polymerase promoter on either side of the ''lsy-2'' gene - 5' TAATACGACTCACTATAGGG 3' | '''Primer Sequence:''' specific to T7 RNA polymerase promoter on either side of the ''lsy-2'' gene - | ||
<br> | 5' TAATACGACTCACTATAGGG 3' | ||
<br><br> | |||
'''PCR Conditions:'''<br> | '''PCR Conditions:'''<br> | ||
{| border="1" | {| border="1" | ||
