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BioMicroCenter:Caliper LabChip GX: Difference between revisions
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[[Image:BioMicroCenter_caliper mRNA.jpg|center|200px]] | [[Image:BioMicroCenter_caliper mRNA.jpg|center|200px]] | ||
'''Caliper RQS VS Agilent RIN ''' | |||
RNA metrics such as peak heights, peak areas, and concentration are used to determine an RQS (RNA Quality Score) number for each sample. The Caliper RQS is a calculated score that rates the quality of RNA samples. The RQS has been validated to correlate well with Agilent’s RIN (RNA Integrity Number) and follows the same 0-10 scale rating. Results comparing RIN to RQS for the same samples run on both LabChip GX and Agilent’s Bioanalyzer 2100 typically show <10% deviation. The RQS is consistent over Caliper’s standard sensitivity RNA assay concentration range (25 to 250 ng/μL) with CV’s <20%. For more detailed information about the Caliper RQS, please refer to ‘RNA Quality Score (RQS) Calculation and Correlation to RIN’. | |||
=== [[BioMicroCenter:Caliper_INFO | Software]] === | === [[BioMicroCenter:Caliper_INFO | Software]] === | ||
The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | ||
