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BioMicroCenter:Caliper LabChip GX: Difference between revisions
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10. Messenger RNA Assay: The RNA contamination ratio is computed. This is the ratio of the area of all the fragments to total RNA area. | 10. Messenger RNA Assay: The RNA contamination ratio is computed. This is the ratio of the area of all the fragments to total RNA area. | ||
'''How to Read Caliper RNA Analysis Result ''' | |||
The Caliper RNA analysis results are almost the same as Agilent Bioanalyzer results. Caliper Life Sciences LabChip GX system analyzes RNA by electrophoretic separation on our microfluidic sipper chips. RNA molecules are separated and subsequently detected via laser induced fluorescence. The LabChip GX Software displays the raw data as an electropherogram and generates a gel-like image for visualization, data such as peak heights, peak areas, concentration, etc., are then calculated and stored as digitized data as text files and tables. These parameters are next used to determine an RQS (RNA Quality Score) number, a calculated score that rates the quality of RNA samples. | |||
• Caliper RNA Ladder Result (Electropherogram of a typical RNA ladder): Total 9 peaks. The first peak is lower marker used to align the ladder data with data from the sample wells. Others represent different sizing ( 200nt, 500nt, 1000nt, 1500nt, 2000nt, 3000nt, 4000nt and 6000nt). | |||
[[Image:BioMicroCenter_caliper ladder3.jpg|center|200px]] | |||
=== [[BioMicroCenter:Caliper_INFO | Software]] === | === [[BioMicroCenter:Caliper_INFO | Software]] === | ||
The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | ||
