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BioMicroCenter:Caliper LabChip GX: Difference between revisions
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[[Image:BioMicroCenter_caliper ladder.jpg|center|200px]] | [[Image:BioMicroCenter_caliper ladder.jpg|center|200px]] | ||
5. The ladder is analyzed and a standard curve of migration time versus RNA size is plotted from the RNA ladder by interpolation between individual RNA fragment size/migration points. | |||
[[Image:BioMicroCenter_caliper ladder2.jpg|center|200px]] | |||
6. The standard curve and the markers are used to calculate RNA fragment sizes for each well from the migration times measured. | |||
7. The Total RNA present is computed by finding the area under the electropherogram trace.The baseline for this integration is a straight line starting at the end of the lower marker and ending at the baseline end time. The height of the baseline endpoints is computed from an average of a five second window around the baseline start and end times. | |||
8. The Total RNA concentration in the sample is computed from the ratio of the RNA area in the sample to the RNA area in the ladder multiplied by the ladder concentration specified in the assay. | |||
9. Assay-defined RNA fragments are identified from the peaks in the peak table. Fragments | |||
are located by finding the largest peak within a size range associated with the fragment. | |||
For Eukaryote RNA assays, 5S, 18S and 28S fragments are located. For Prokaryote | |||
assays, 5S, 16S and 23S fragments are identified. | |||
=== [[BioMicroCenter:Caliper_INFO | Software]] === | === [[BioMicroCenter:Caliper_INFO | Software]] === | ||
The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | ||
