BIOL388/S19:Class Journal Week 11: Difference between revisions

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BIOL388/S19:Class Journal Week 11 (view source)

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→‎Austin Dias Reflection: saved reflection
(→‎Austin Dias Reflection: saved question 2)
(→‎Austin Dias Reflection: saved reflection)
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==[[User:Austindias|Austin Dias]] Reflection ==
==[[User:Austindias|Austin Dias]] Reflection ==
#Discuss amongst yourselves whether or not the Tai et al (2007) journal club paper gives you enough information to perform such a simulation.
1. Discuss amongst yourselves whether or not the Tai et al (2007) journal club paper gives you enough information to perform such a simulation.
#What parameters are explicitly given?
 
2. What parameters are explicitly given?


The researchers only explicitly provide the dilution rate and the volume in the container. Searching back through their previous paper is necessary to find nutrient concentrations. The parameters that were explicitly stated are listed below:  
The researchers only explicitly provide the dilution rate and the volume in the container. Searching back through their previous paper is necessary to find nutrient concentrations. The parameters that were explicitly stated are listed below:  
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*** 46 g liter^1 glucose
*** 46 g liter^1 glucose


#Can you back any others out from the data tables?
3. Can you back any others out from the data tables?
#To what in the model do the quantities in Table 1 correspond?
Table 1 provides dilution rates for each experimental set up. While Table 2 provides cell biomass and nutrient mass values.
 
 


A temperature probe connected to a cryostat controlled cultures grown at 12°C.
4. To what in the model do the quantities in Table 1 correspond?
Cultures were grown in a defined synthetic medium that was limited by
carbon or by nitrogen with all other growth requirements in excess as previously described (Tai et al., 2005). The dilution rate was set at 0.03 h1 with pH
measured on-line and kept constant at 5.0 by automatic addition of 2 M KOH
using an Applikon ADI 1030 Biocontroller. The stirrer speed was set to 600
rpm. Anaerobic growth and steady-state conditions were maintained as previously described (Tai et al., 2005). Biomass dry weight, metabolites, dissolved
oxygen, and gas profiles were constant for at least three volume changes
before sampling.


==[[User:EdwardRyanTalatala|Edward's Journal Reflection]]==
==[[User:EdwardRyanTalatala|Edward's Journal Reflection]]==
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